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Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48258

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG2a Kappa/IgG1 Kappa Clone # A103 + T311 + HMB45

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Localization

Cytoplasmic

Specificity

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG2a Kappa/IgG1 Kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - IHC-Prediluted [NBP2-48258]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - IHC-Prediluted [NBP2-48258]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - IHC-Prediluted [NBP2-48258] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)

Alternate Names

MART1, MART-1, melan-A, MLANA

Gene Symbol

MLANA

Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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