Mesothelin Antibody (MB-G10)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85538

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Porcine

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, SDS-Page, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MB-G10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mesothelin Antibodies »

Product Specifications

Immunogen

This Mesothelin Antibody (MB-G10) was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human Mesothelin full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. (Uniprot: Q13421)

Reactivity Notes

Cross reactivity with homologues from other sources has not been tested

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This product was purified from tissue culture supernatant fluid by Protein A chromatography

Store antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Mesothelin Antibody (MB-G10)

Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538]

Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538]

Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.
Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538]

Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538]

Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538] - Immunohistochemistry using Mesothelin antibody (MB-G10) to react with two epitopes on Mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-Mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to Equineradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.
SDS-PAGE: Mesothelin Antibody (MB-G10) [NB110-85538]

SDS-PAGE: Mesothelin Antibody (MB-G10) [NB110-85538]

SDS-Page: Mesothelin Antibody (MB-G10) [NB110-85538] - SDS-PAGE of Mesothelin antibody (MB-G10). Lane 1: Non-Reduced Mesothelin antibody (MB-G10). Lane M: 3 uL OPAL Pre-stained Marker. Lane 2: Reduced Mesothelin antibody (MB-G10). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.
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Applications for Mesothelin Antibody (MB-G10)

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For Anti-mesothelin immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mesothelin

Mesothelin is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2-terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human)

Gene Symbol

MSLN

UniProt

Q13421

Additional Mesothelin Products

Product Documents for Mesothelin Antibody (MB-G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mesothelin Antibody (MB-G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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