Mesothelin Antibody (MB-G10) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85538CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB110-85538

Alexa Fluor 350

NB110-85538AF350

Alexa Fluor 405

NB110-85538AF405

Alexa Fluor 488

NB110-85538AF488

Alexa Fluor 532

NB110-85538AF532

Alexa Fluor 594

NB110-85538AF594

Alexa Fluor 647

NB110-85538AF647

Alexa Fluor 700

NB110-85538AF700

Alexa Fluor 750

NB110-85538AF750

Allophycocyanin

NB110-85538APC

Biotin

NB110-85538B

DyLight 350

NB110-85538UV

DyLight 405

NB110-85538V

DyLight 488

NB110-85538G

DyLight 550

NB110-85538R

DyLight 594

NB110-85538DL594

DyLight 650

NB110-85538C

DyLight 680

NB110-85538FR

DyLight 755

NB110-85538IR

FITC

NB110-85538F

HRP

NB110-85538H

Janelia Fluor 525

NB110-85538JF525

Janelia Fluor 549

NB110-85538JF549

Janelia Fluor 585

NB110-85538JF585

Janelia Fluor 635

NB110-85538JF635

Janelia Fluor 646

NB110-85538JF646

Janelia Fluor 669

NB110-85538JF669

mFluor Violet 450 SE

NB110-85538MFV450

mFluor Violet 500 SE

NB110-85538MFV500

mFluor Violet 610 SE

NB110-85538MFV610

PE

NB110-85538PE

PerCP

NB110-85538PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, SDS-Page, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG_2A Clone # MB-G10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mesothelin Antibodies »

Product Summary for Mesothelin Antibody (MB-G10) [CoraFluor™ 1]

Immunogen

This Mesothelin Antibody (MB-G10) was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human Mesothelin full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein. (Uniprot: Q13421)

Reactivity Notes

Cross reactivity with homologues from other sources has not been tested

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Mesothelin Antibody (MB-G10) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

SDS-Page

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Mesothelin

MSLN, also known as Mesothelin, has 4 isoforms, a 630 amino acid isoform that is 69 kDa, a 622 amino acid isoform that is 68 kDa, a 656 amino acid isoform that is 71 kDa, and a 621 amino acid isoform that is 68 kDa, expressed in lung, heart, placenta kidney, and overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas. Membrane-anchored forms may play a role in cellular adhesion and megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Current research is being performed on several diseases and disorders including squamous cell carcinoma, ovarian cancer, carcinoma, malignant biphasic mesothelioma, bile duct adenoma, malignant mesothelioma, malignant pleural mesothelioma, gastrointestinal stromal tumor, renal clear cell carcinoma, epithelioid sarcoma, benign mesothelioma, pancreatic ductal adenocarcinoma, asbestosis, pancreatic ductal carcinoma, thymic carcinoma, acute myeloid leukemia, epithelial ovarian cancer, myeloid leukemia, thoracic cancer, and pancreatitis. This protein has also shown an interaction with MUC16 and RSG1 in cell adhesion and pancreas development processes and pathways.

Alternate Names

CAK1, MPF, MSLN, SMR

Gene Symbol

MSLN

Additional Mesothelin Products

Product Documents for Mesothelin Antibody (MB-G10) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Mesothelin Antibody (MB-G10) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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