mGluR5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13190

Novus Biologicals, part of Bio-Techne
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NBP3-13190
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all mGluR5 Antibodies »

Product Summary for mGluR5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse mGluR5. The exact sequence is proprietary.

Reactivity Notes

Human (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for mGluR5 Antibody

Western Blot: mGluR5 Antibody [NBP3-13190]

Western Blot: mGluR5 Antibody [NBP3-13190]

Western Blot: mGluR5 Antibody [NBP3-13190] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody (NBP3-13190) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: mGluR5 Antibody [NBP3-13190]

Immunocytochemistry/ Immunofluorescence: mGluR5 Antibody [NBP3-13190]

Immunocytochemistry/Immunofluorescence: mGluR5 Antibody [NBP3-13190] - mGluR5 antibody detects mGluR5 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mGluR5 stained by mGluR5 antibody (NBP3-13190) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for mGluR5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mGluR5

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Class I metabotropic glutamate receptors (mGluRs) have been postulated to play a role in synaptic plasticity. It has been suggested that mGluR5 may be the primary high affinity ACPD receptor in CA1 neurons (1). Also, neuroprotection by mGluR system has been linked to the modulation of both the free radical nitric oxide (NO) and programmed cell death (PCD). Activation of group I mGluR subtypes utilized an effective, "upstream" mechanism for the inhibition of cysteine protease activity that offered an enhanced level of neuroprotection through both the preservation of genomic DNA integrity and the maintenance of PS membrane asymmetry (2). It has also been shown that activation of mGluRs enhances formation of an mGluR-Homer-PIKE-L complex, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Our findings indicate that this complex mediates the well-known ability of agonists of mGluR5 to prevent neuronal apoptosis (3).

Long Name

Metabotropic Glutamate Receptor 5

Alternate Names

GPRC1E, GRM5, mGlu5

Gene Symbol

GRM5

Additional mGluR5 Products

Product Documents for mGluR5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mGluR5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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