MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42997
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # M5/114.15.2
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was MHC Class II.
Reactivity Notes
Use in Human reported in scientific literature (PMID:29990503).
Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]
Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [Allophycocyanin] [NBP1-42997] - Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right). Cells in the lymphocyte gate were used for analysis.
Applications for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Application Notes
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MHC class II (I-A/I-E)
Long Name
Major Histocompatibility Complex Class II IA and IE
Alternate Names
H2-Ea, MHC class II IA/IE
Gene Symbol
H2-Ea
Additional MHC class II (I-A/I-E) Products
Product Documents for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]
Product Specific Notices for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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