MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Atto594]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43312PEATT594
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry
Label
PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # M5/114.15.2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen for this antibody was MHC Class II.
Reactivity Notes
Use in Human reported in scientific literature (PMID:29990503).
Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Applications for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Atto594]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: MHC class II (I-A/I-E)
Long Name
Major Histocompatibility Complex Class II IA and IE
Alternate Names
H2-Ea, MHC class II IA/IE
Gene Symbol
H2-Ea
Additional MHC class II (I-A/I-E) Products
Product Documents for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Atto594]
Product Specific Notices for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Atto594]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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