Key Product Details

Species Reactivity

Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 525

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MHC Class II RT1B Antibodies »

Product Specifications

Immunogen

Rat thymocyte membrane glycoproteins

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NB100-65541 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. This antibody does not react with C57BL6 or C57BL6/j mice because they are haplotype b. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with Rat BDIX strain. Please be aware of the possible size variance seen in western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 525]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Free-Floating

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MHC Class II RT1B

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Alternate Names

DMA, DMB, DP beta 1, DPB 1, DPB1, DRB, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DPB1, HLADM, HLADP1B, Major histocompatibility complex class II, Major histocompatibility complex class II DP beta 1, MHC class II antigen DMB, MHC DPB1, RING6, RING7

Gene Symbol

RT1-B

Additional MHC Class II RT1B Products

Product Documents for MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 525]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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