MHC Class II RT1B Antibody (OX-6) [DyLight 405]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65541V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MHC Class II RT1B Antibody (OX-6) [DyLight 405]
Immunogen
Rat thymocyte membrane glycoproteins
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
NB100-65541 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. This antibody does not react with C57BL6 or C57BL6/j mice because they are haplotype b. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with Rat BDIX strain. Please be aware of the possible size variance seen in western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for MHC Class II RT1B Antibody (OX-6) [DyLight 405]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MHC Class II RT1B
Alternate Names
DMA, DMB, DP beta 1, DPB 1, DPB1, DRB, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DPB1, HLADM, HLADP1B, Major histocompatibility complex class II, Major histocompatibility complex class II DP beta 1, MHC class II antigen DMB, MHC DPB1, RING6, RING7
Gene Symbol
RT1-B
Additional MHC Class II RT1B Products
Product Documents for MHC Class II RT1B Antibody (OX-6) [DyLight 405]
Product Specific Notices for MHC Class II RT1B Antibody (OX-6) [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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