MIG2/Kindlin-2 Antibody (OTI9E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47745

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E4

Concentration

1 mg/ml

View all MIG2 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

77.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MIG2/Kindlin-2 Antibody (OTI9E4)

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - GST-tagged wild-type MIG2/Kindlin-2 or MIG2/Kindlin-2 (S159A) were incubated without IKKE (-), or with purified wild-type IKKE (WT) or mutant IKKE (K38A) in kinase buffer and resolved by SDS-PAGE, followed by immunoblotting analysis with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019159/) licensed under a CC-BY license
Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745]

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.
View 9 more images

Applications for MIG2/Kindlin-2 Antibody (OTI9E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIG2

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Long Name

Mitogen Inducible Gene 2

Alternate Names

FERMT2, KIND2, Kindlin-2, PLEKHC1

Entrez Gene IDs

10979 (Human)

Gene Symbol

FERMT2

Additional MIG2 Products

Product Documents for MIG2/Kindlin-2 Antibody (OTI9E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MIG2/Kindlin-2 Antibody (OTI9E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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