MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72687
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI9E4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
77.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4)Azide and BSA Free [NBP2-72687]
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]
Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]
Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.Applications for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:150
Immunoprecipitation
2ug/500ul
Western Blot
1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MIG2
Long Name
Mitogen Inducible Gene 2
Alternate Names
FERMT2, KIND2, Kindlin-2, PLEKHC1
Gene Symbol
FERMT2
Additional MIG2 Products
Product Documents for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free
Product Specific Notices for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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