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MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72687

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

77.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4)Azide and BSA Free [NBP2-72687]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4)Azide and BSA Free [NBP2-72687]

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.
Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.
Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]

Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687]

Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.

Applications for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIG2

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Long Name

Mitogen Inducible Gene 2

Alternate Names

FERMT2, KIND2, Kindlin-2, PLEKHC1

Gene Symbol

FERMT2

Additional MIG2 Products

Product Documents for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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