MITF Antibody (21D1418) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56561

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 21D1418

Format

BSA Free

Concentration

1.0 mg/ml

View all MITF Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to the 300-350 a.a. region of accession number O75030.

Specificity

Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed. Users are encouraged to utilize the peptide immunogen sequence to gain further information about the isoforms recognized by the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MITF Antibody (21D1418) - BSA Free

Western Blot: MITF Antibody (21D1418)BSA Free [NB100-56561]

Western Blot: MITF Antibody (21D1418)BSA Free [NB100-56561]

Western Blot: MITF Antibody (21D1418) [NB100-56561] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: MITF Antibody (21D1418) - BSA Free [NB100-56561]

Immunocytochemistry/ Immunofluorescence: MITF Antibody (21D1418) - BSA Free [NB100-56561]

Immunocytochemistry/Immunofluorescence: MITF Antibody (21D1418) [NB100-56561] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MITF Antibody (21D1418) NB100-56561 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - BSA Free [NB100-56561]

Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - BSA Free [NB100-56561]

Immunohistochemistry-Paraffin: MITF Antibody (21D1418) [NB100-56561] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of MITF (21D1418) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. Some nuclear reactivity is observed as well.
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Applications for MITF Antibody (21D1418) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-5 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

0.1 - 1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MITF

Microphthalmia-associated bHLH-LZ transcription factor (Mitf) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC transcription factors. Mitf can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. Mitf is expressed as multiple isoforms, which share common transcription activation and DNA-binding domains, but differ in the amino-terminal region. Mitf is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Entrez Gene IDs

4286 (Human); 17342 (Mouse); 25094 (Rat)

Gene Symbol

MITF

UniProt

O75030 (Human)

Additional MITF Products

Product Documents for MITF Antibody (21D1418) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MITF Antibody (21D1418) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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