MITF Antibody (21D1418) - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33229
![Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]](https://resources.bio-techne.com/images/products/MITF-Antibody-21D1418-Azide-Free-Western-Blot-NBP2-33229-img0002.jpg "Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 21D1418
Format
Azide Free
Concentration
1.0 mg/ml
Product Summary for MITF Antibody (21D1418) - Azide Free
Immunogen
A synthetic peptide mapping to the 300-350 a.a. region of accession number O75030.
Specificity
Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed. Users are encouraged to utilize the peptide immunogen sequence to gain further information about the isoforms recognized by the antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MITF Antibody (21D1418) - Azide Free
Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]
Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).![Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229]](https://resources.bio-techne.com/images/products/MITF-Antibody-21D1418-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33229-img0003.jpg "Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229]")
Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229]
Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - MITF Antibody (21D1418) [NB100-56561] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of MITF (21D1418) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. Some nuclear reactivity is observed as well.![Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]](https://resources.bio-techne.com/images/products/MITF-Antibody-21D1418-Azide-Free-Western-Blot-NBP2-33229-img0001.jpg "Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]")
Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]
Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.Applications for MITF Antibody (21D1418) - Azide Free
Application
Recommended Usage
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
0.1-5ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS - BSA free
Format
Azide Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MITF
Long Name
Microphthalmia-associated Transcription Factor
Alternate Names
bHLHe32, MI, WS2A
Gene Symbol
MITF
Additional MITF Products
Product Documents for MITF Antibody (21D1418) - Azide Free
Product Specific Notices for MITF Antibody (21D1418) - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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