Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM198

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Mitochondria Antibodies »

Product Specifications

Immunogen

Semi-Purified mitochondrial preparation

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Mitochondria in cytoplasm

Specificity

monoclonal antibody 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Marker

Mitochondrial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32981).

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Mitochondria Antibody (SPM198) - Azide and BSA Free

Western Blot: Mitochondria Antibody (SPM198)Azide and BSA Free [NBP2-34745]

Western Blot: Mitochondria Antibody (SPM198)Azide and BSA Free [NBP2-34745]

Western Blot: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745] - Western Blot Analysis of HeLa cell lysate using Mitochondria Antibody (SPM198).
Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745]

Immunocytochemistry/ Immunofluorescence: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745]

Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745] - Confocal immunofluorescence image of HeLa cells using Mitochondria Antibody (SPM198) labeled in Green (CF488) and Red Dot is used to label the nuclei purple.
Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745]

Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745]

Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - Azide and BSA Free [NBP2-34745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).

Applications for Mitochondria Antibody (SPM198) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Mitochondria

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Additional Mitochondria Products

Product Documents for Mitochondria Antibody (SPM198) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Mitochondria Antibody (SPM198) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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