Mitochondria Antibody (SPM198) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45204
![Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - IHC-Prediluted [NBP2-45204]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-SPM198-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45204-img0001.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - IHC-Prediluted [NBP2-45204]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM198
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Semi-Purified mitochondrial preparation
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Mitochondria in cytoplasm
Specificity
monoclonal antibody 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Mitochondria Antibody (SPM198) - IHC-Prediluted
Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - IHC-Prediluted [NBP2-45204]
Immunohistochemistry-Paraffin: Mitochondria Antibody (SPM198) - IHC-Prediluted [NBP2-45204] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).Applications for Mitochondria Antibody (SPM198) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (SPM198) - IHC-Prediluted
Product Specific Notices for Mitochondria Antibody (SPM198) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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