MKK7/MEK7 Antibody (1T3M6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15456

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15456-100ul
NBP3-15456-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T3M6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MKK7 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 320-419 of human MKK7/MEK7 (O14733). FPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPHLPFFR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK7/MEK7 Antibody (1T3M6)

Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456]

Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456]

Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456] - Western blot analysis of extracts of various cell lines, using MKK7/MEK7 Rabbit mAb (NBP3-15456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456]

Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456]

Western Blot: MKK7/MEK7 Antibody (1T3M6) [NBP3-15456] - Western blot analysis of extracts of Rat heart, using MKK7/MEK7 Rabbit mAb (NBP3-15456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for MKK7/MEK7 Antibody (1T3M6)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK7

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Long Name

Mitogen-activated Protein Kinase Kinase 7

Alternate Names

JNKK2, MAP2K7, MKE7, PRKMK7

Gene Symbol

MAP2K7

Additional MKK7 Products

Product Documents for MKK7/MEK7 Antibody (1T3M6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK7/MEK7 Antibody (1T3M6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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