MKK7/MEK7 [p Thr277] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29678

Novus Biologicals, part of Bio-Techne
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NBP2-29678
NBP2-29678-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MKK7 Antibodies »

Product Specifications

Immunogen

peptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4.

Modification

p Thr277

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_145185.2

Scientific Data Images for MKK7/MEK7 [p Thr277] Antibody

Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678]

Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678]

Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678] - (No peptide/Lane 1), A431 treated with Calyculin A and okadaic acid (Control peptide/Lane 2), A431 treated with Calyculin A and okadaic acid (Phosphopeptide (Thr275/Ser277)/Lane 3) lysates were probed Anti-Phospho-MKK7/SAPKK4 (Thr275/Ser277) (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Phospho-MKK7/SAPKK4 (Thr275/Ser277) (~47 kDa).
Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678]

Western Blot: MKK7/MEK7 [p Thr277] Antibody [NBP2-29678]

Western Blot: MEK7 [p Thr277] Antibody [NBP2-29678] - Recombinant MKK7b1 (14-542) was left untreated (Lane 1) or phosphorylated by MEKK1 (14-196) (Lane 2) and probed with anti-phospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).

Applications for MKK7/MEK7 [p Thr277] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Tris-Glycine (phH 7.4), 0.15M NaCl and 30% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKK7

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Long Name

Mitogen-activated Protein Kinase Kinase 7

Alternate Names

JNKK2, MAP2K7, MKE7, PRKMK7

Gene Symbol

MAP2K7

UniProt

O14733

Additional MKK7 Products

Product Documents for MKK7/MEK7 [p Thr277] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK7/MEK7 [p Thr277] Antibody

Relevant Entrez Gene Numbers: NM_145185.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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