MMP-3 Antibody (MMP3/1994R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07322

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-07322-100ug
NBP3-07322-20ug
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # MMP3/1994R

Concentration

0.2 mg/ml

View all MMP-3 Antibodies »

Product Summary for MMP-3 Antibody (MMP3/1994R)

Immunogen

Recombinant fragment (around aa 317-327) of human MMP-3 protein (Uniprot: P08254)

Localization

Cytoplasm, extracellular matrix

Marker

Marker of Metastasis and Rheumatoid Arthritis

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08627)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for MMP-3 Antibody (MMP3/1994R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MMP-3

Effective inhibitors of matrix metalloproteinases (MMPs), a family of connective tissue-degrading enzymes, could be useful for the treatment of diseases such as cancer, multiple sclerosis, and arthritis (1). Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. MMP3 (stromelysin) is a secreted metalloprotease with a wide range of substrate specificities. MMP3 is synthesized in a preproenzyme form with a calculated size of 54 kDa and a 17-amino acid long signal peptide. Data indicate that the expression and the possible involvement of secreted metalloproteases in tumorigenesis result from a specific interaction between the transforming factor and the target cell, which may vary in different species (2). In neural studies, matrix metalloproteinase-3 (MMP-3) was newly induced and activated in stressed dopamine cells, and the active form of MMP-3 (actMMP-3) was released into the medium. The released actMMP-3, as well as catalytically active recombinant MMP-3 (cMMP-3) led to microglial activation and superoxide generation in microglia and enhanced DA cell death. (3).

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Gene Symbol

MMP3

UniProt

P08254

Additional MMP-3 Products

Product Documents for MMP-3 Antibody (MMP3/1994R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-3 Antibody (MMP3/1994R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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