Mouse 4-1BB/TNFRSF9/CD137 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB937S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 158332
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
Val24-Leu187
Accession # P20334
Val24-Leu187
Accession # P20334
Specificity
Detects mouse 4-1BB in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, recombinant mouse (rm) CD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTROY, rmTROY, rhTNF sRI, rhTNF sRII, or rmTNF sRII.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse 4-1BB/TNFRSF9/CD137 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Activated and resting mouse splenocytes
Sample: Activated and resting mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: 4-1BB/TNFRSF9/CD137
4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region (1 - 3). A soluble 4-1BB is released from surfaces of cells expressing the transmembrane protein (4). Mouse 4‑1BB shares approximately 60% aa sequence identity with its human counterpart. 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils (5). The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4-1BBL binding, 4-1BB transduce a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4-1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DC-activating molecules that enhances cytokine production and upregulates expression of B7-1 and B7‑2 costimulatory molecules, resulting in an improved ability to stimulate T cell responses (1 - 5).
References
- Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Kwon, B.S. and S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963.
- Wilcox, R.A. et al. (2002) J. Immunol. 168:4262.
- Kwon, B. et al. (2002) TRENDS in Immunology 23:378.
Alternate Names
41BB, CD137, ILA, TNFRSF9
Gene Symbol
TNFRSF9
UniProt
Additional 4-1BB/TNFRSF9/CD137 Products
Product Documents for Mouse 4-1BB/TNFRSF9/CD137 Alexa Fluor® 750-conjugated Antibody
Product Specific Notices for Mouse 4-1BB/TNFRSF9/CD137 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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