Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4960
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Angiopoietin-like Protein 7/ANGPTL7
Gln22-Pro337
Accession # Q8R1Q3
Gln22-Pro337
Accession # Q8R1Q3
Specificity
Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ANGPTL7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Detection of Rat Angiopoietin-like Protein 7/ANGPTL7 by Western Blot.
Western blot shows lysates of rat eye tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Angiopoietin-like Protein 7/ANGPTL7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat eye tissue
Sample: Rat eye tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 7/ANGPTL7
Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.
Long Name
Angiopoietin-like Protein 7
Alternate Names
ANGPTL7, AngX, CDT6
Entrez Gene IDs
Gene Symbol
ANGPTL7
UniProt
Additional Angiopoietin-like Protein 7/ANGPTL7 Products
Product Documents for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Product Specific Notices for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
For research use only
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