Mouse B220/CD45R Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1217F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Product Specifications
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Specificity
Detects mouse B220/CD45R.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse B220/CD45R Fluorescein-conjugated Antibody
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse B220/CD45R Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using FAB1217F in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
References
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for Mouse B220/CD45R Fluorescein-conjugated Antibody
Product Specific Notices for Mouse B220/CD45R Fluorescein-conjugated Antibody
For research use only
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