Mouse Bad N-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB850
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 64103
Product Specifications
Immunogen
E. coli-derived recombinant mouse Bad
Specificity
Detects endogenous mouse Bad in Western blots. In Western blots, this antibody does not cross-react with human Bad. The antibody also detects a 32 kDa band in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Bad N-Terminus Antibody
Detection of Mouse Bad N-Terminus by Western Blot.
Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Bad N-Terminus at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Bad in NIH‑3T3 Mouse Cell Line.
Bad was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Bad N-Terminus Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Western Blot
1 µg/mL
Sample: CTLL-2 mouse cytotoxic T cell line and L-929 mouse fibroblast cell line
Sample: CTLL-2 mouse cytotoxic T cell line and L-929 mouse fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB850 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bad
Long Name
Bcl-xL/Bcl-2 Associated Death Promoter
Alternate Names
BBC6, BCL2L8
Gene Symbol
BAD
Additional Bad Products
Product Documents for Mouse Bad N-Terminus Antibody
Product Specific Notices for Mouse Bad N-Terminus Antibody
For research use only
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