Mouse BAMBI/NMA Antibody

Detection of Mouse BAMBI/NMA by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse BAMBI/NMA by Western Blot

Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse BAMBI/NMA by Western Blot

Knockdown of BAMBI suppressed Wnt/ beta-catenin signaling. All the cell samples were harvested after transfection and myogenic induction for 48 h. (a) The western blot images of nuclear beta-catenin and laminB1; (b) the nuclear beta-catenin protein levels; (c) the mRNA expression of Axin2; (d) the western blot images of nuclear beta-catenin and laminB1; (e) the nuclear beta-catenin protein levels; (f) the mRNA expression of Axin2; (g) the western blot images of BAMBI and GAPDH; and (h) the mRNA and protein expression of BAMBI. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.