Mouse Cathepsin X/Z/P Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1033
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
Ala23-Val306
Accession # Q9WUU7
Ala23-Val306
Accession # Q9WUU7
Specificity
Detects mouse Cathepsin X/Z/P in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Cathepsin X/Z/P is observed and less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin-D, rmCathepsin-H, rmCathepsin-L, rmCathepsin‑C, and rmCathepsin-B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Cathepsin X/Z/P Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin X/Z/P
Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).
References
- Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
- Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
- Nagler and Menard (1998) FEBS Lett. 434:135.
- Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
- Pungercar, et al. (2000) Pflugers Arch. 439:R119.
- Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
- Menard, et al. (2001) Biol. Chem. 382:839.
- Therrien, et al. (2001) Biochemistry 40:2702.
- Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
- Guncar, et al. (2000) Structure Fold Des. 8:305.
- Lustigman, et al. (1996) J. Biol. Chem. 271:30181.
Alternate Names
Cathepsin P, Cathepsin Z, CTSZ
Gene Symbol
CTSZ
UniProt
Additional Cathepsin X/Z/P Products
Product Documents for Mouse Cathepsin X/Z/P Biotinylated Antibody
Product Specific Notices for Mouse Cathepsin X/Z/P Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...