Mouse Cathepsin X/Z/P Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1033
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Neutralization, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin X/Z/P
Ala23-Val306
Accession # Q9WUU7
Ala23-Val306
Accession # Q9WUU7
Specificity
Detects mouse Cathepsin X/Z/P in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Cathepsin X/Z/P Antibody
Cathepsin X/Z/P in Mouse Splenocytes.
Cathepsin X/Z/P was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1033) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse Cathepsin X/Z/P Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
Sample: Recombinant Mouse Cathepsin X/Z/P (Catalog # 1033-CY)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin X/Z/P
Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).
References
- Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
- Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
- Nagler and Menard (1998) FEBS Lett. 434:135.
- Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
- Pungercar, et al. (2000) Pflugers Arch. 439:R119.
- Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
- Menard, et al. (2001) Biol. Chem. 382:839.
- Therrien, et al. (2001) Biochemistry 40:2702.
- Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
- Guncar, et al. (2000) Structure Fold Des. 8:305.
- Lustigman, et al. (1996) J. Biol. Chem. 271:30181.
Alternate Names
Cathepsin P, Cathepsin Z, CTSZ
Gene Symbol
CTSZ
UniProt
Additional Cathepsin X/Z/P Products
Product Documents for Mouse Cathepsin X/Z/P Antibody
Product Specific Notices for Mouse Cathepsin X/Z/P Antibody
For research use only
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