Mouse CCL1/I-309/TCA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8451
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
ELISA Development, Functional Assay, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 148107
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I‑309/TCA‑3
Lys24-Cys92
Accession # P10146
Lys24-Cys92
Accession # P10146
Specificity
Detects mouse CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL1, recombinant mouse (rm) CCL17, rmCCL19 or rmCCL24 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL1/I-309/TCA-3 Antibody
Chemotaxis Induced by CCL1/I-309/TCA‑3 and Neutralization by Mouse CCL1/I-309/TCA‑3 Antibody.
Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL1/I-309/ TCA-3 Monoclonal Antibody (Catalog # MAB8451). The ND50 is typically 0.6-3.0 µg/mL.Applications for Mouse CCL1/I-309/TCA-3 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL1/I-309/TCA-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL1/I-309/TCA-3.
Mouse CCL1/TCA-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB8451 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3
Mouse TCA‑3 is a member of the CC beta family of chemokines, designated CCL1. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, is the orthologue of mouse TCA‑3. Mouse TCA‑3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.
TCA‑3 cDNA encodes a 92 aa precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA‑3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA‑3 has been shown to chemoattract T-cells.
References
- Goya, I. et al. (1998) J. Immunol. 160:1975.
- Burd, P. et al. (1987) J. Immunol. 139:3126.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Mouse CCL1/I-309/TCA-3 Antibody
Product Specific Notices for Mouse CCL1/I-309/TCA-3 Antibody
For research use only
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