Mouse CCL1/I-309/TCA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB845
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
ELISA Development, In vivo assay, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 148113
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3
Lys24-Cys92
Accession # P10146
Lys24-Cys92
Accession # P10146
Specificity
Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton
rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17,
18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5 ,6, 7, 8,
9/10/MIP-1 gamma, 11,
12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL1/I-309/TCA-3 Antibody
Chemotaxis Induced by CCL1/ITCA-3 and Neutralization by Mouse CCL1/ITCA-3 Antibody.
Recombinant Mouse CCL1/TCA-3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA-3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA-3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL.Applications for Mouse CCL1/I-309/TCA-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL1/I-309/TCA-3 (Catalog # 845-TC)
Sample: Recombinant Mouse CCL1/I-309/TCA-3 (Catalog # 845-TC)
Neutralization
Measured by its ability to neutralize CCL1/I-309/TCA-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I-309/TCA-3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3
Mouse CCL1, also known as TCA-3, is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.
CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.
References
- Goya, I. et al. (1998) J. Immunol. 160:1975.
- Burd, P. et al. (1987) J. Immunol. 139:3126.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Mouse CCL1/I-309/TCA-3 Antibody
Product Specific Notices for Mouse CCL1/I-309/TCA-3 Antibody
For research use only
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