Mouse CCL1/I-309/TCA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF845
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization, Western Blot
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3
Lys24-Cys92
Accession # P10146
Lys24-Cys92
Accession # P10146
Specificity
Detects mouse CCL1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL1/I-309/TCA-3 Antibody
Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody.
Recombinant Mouse CCL1/ TCA-3 (Catalog # 845-TC) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/ TCA-3 (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CCL1/TCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF845). The ND50 is typically 0.3-1.5 µg/mL.Applications for Mouse CCL1/I-309/TCA-3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL1/I-309/TCA-3 (Catalog # 845-TC)
Sample: Recombinant Mouse CCL1/I-309/TCA-3 (Catalog # 845-TC)
Neutralization
Measured by its ability to neutralize CCL1/I-309/TCA-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I-309/TCA-3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF845 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3
Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.
TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T cells.
References
- Goya, I. et al. (1998) J. Immunol. 160:1975.
- Burd, P. et al. (1987) J. Immunol. 139:3126.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Mouse CCL1/I-309/TCA-3 Antibody
Product Specific Notices for Mouse CCL1/I-309/TCA-3 Antibody
For research use only
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