HEK293 human embryonic kidney cell line transfected with (A) mouse CCR10 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815) or Rat IgG_2AIsotype Control (Catalog # MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR10 in Raw264.7 cells by Flow Cytometry.

Raw264.7 cells were stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR10 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR10 and eGFP; Raw264.7 mouse monocyte/macrophage cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Alternate Names

CCR10, GPR2

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Gene Symbol

CCR10

UniProt

NP_031747

Additional CCR10 Products

Product Documents for Mouse CCR10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCR10 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCR10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2815

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CCR10 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CCR10 Antibody

Detection of CCR10 in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP by Flow Cytometry.