CD117/c-kit was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # MAB1356) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to Bergmann glial cells located around Purkinje cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse CD117/c-kit Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Lineage depleted mouse bone marrow cells

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD117/c-kit under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

SCF R is also known as the c-kit proto-oncogene protein and as CD117. Together with its ligand, stem cell factor (also called c-kit ligand or mast cell growth factor), SCF R plays an essential role in gametogenesis, melanogenesis, and hematopoiesis. SCF R is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitors, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF R expression has also been reported in several human tumor cell lines (1,2).

References

Broudy, V. (1997) Blood 90:1345.
Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Mouse CD117/c-kit Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CD117/c-kit Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD117/c-kit Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1356

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD117/c-kit Antibody

CD117/c-kit in Mouse Brain.