Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB1356A) and Rat Anti-Mouse Sca-1/Ly6 PE-conjugated Monoclonal Antibody (Catalog # FAB1226P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD117/c-kit APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Lineage depleted C57 bone marrow cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD117/c-kit

SCF R is also known as the c-kit proto-oncogene protein and as CD117. Together with its ligand, stem cell factor (also called c-kit ligand or mast cell growth factor), SCF R plays an essential role in gametogenesis, melanogenesis, and hematopoiesis. SCF R is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitors, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF R expression has also been reported in several human tumor cell lines (1,2).

References

Broudy, V. (1997) Blood 90:1345.
Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Mouse CD117/c-kit APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse CD117/c-kit APC-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse CD117/c-kit APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1356A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD117/c-kit APC-conjugated Antibody

Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.