bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

CD151 in bEnd.3 Mouse Cell Line.

CD151 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse CD151 Monoclonal Antibody (Catalog # MAB4609) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse CD151 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: bEnd.3 mouse endothelioma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD151

CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha3/ beta1, alpha6/ beta1, alpha6/ beta4, and alpha7/ beta1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94%-96% aa sequence identity with human and rat CD151.

Alternate Names

CD151, PETA-3, SFA-1, TSPAN24

Entrez Gene IDs

977 (Human); 12476 (Mouse); 64315 (Rat)

Gene Symbol

CD151

UniProt

BAA22447

Additional CD151 Products

Product Documents for Mouse CD151 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD151 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD151 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4609

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD151 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD151 Antibody

Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry.