Mouse CD200 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3355
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD200
Gln31-Gly232
Accession # O54901
Gln31-Gly232
Accession # O54901
Specificity
Detects mouse CD200 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human CD200 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CD200 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD)
Sample: Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD200
CD200, also known as OX-2, is a 45 kDa type I transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The mouse CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-likeV-type and one Ig-like C2-type domain (3). Splice variants of CD200 have been described in human but not in mouse. Within the ECD, mouse CD200 shares 76% and 94% aa sequence identity with human and rat CD200, respectively.
References
Alternate Names
CD200, MOX1, MOX2, MRC, OX-2
Gene Symbol
CD200
UniProt
Additional CD200 Products
Product Documents for Mouse CD200 Biotinylated Antibody
Product Specific Notices for Mouse CD200 Biotinylated Antibody
For research use only
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