Mouse CD30/TNFRSF8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8521

R&D Systems, part of Bio-Techne
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MAB8521
MAB8521-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Western Blot

Cited:

Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 115705

View all CD30/TNFRSF8 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD30/TNFRSF8
Phe19-Thr281
Accession # Q60846

Specificity

Detects mouse CD30/TNFRSF8 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) TNF RI/TNFRSF1A, recombinant human (rh) TNF RI/TNFRSF1A, rhTNF RII/TNFRSF1B, rmTNF‑ alpha/TNFSF2, rhTNF‑ alpha/TNFSF2, rhTNF-beta /TNFSF1, rhCD30/TNFRSF8, or rmCD30 Ligand/TNFSF8 is observed. In Western blots, no cross-reactivity with rhNGFR/TNFRSF16, rmOPG/TNFRSF11B, rmRANK/TNFRSF11A, rmTNF RI/TNFRSF1A, rmTNF RII/TNFRSF1B, rmEDAR, rmTAJ/TNFSF19, rmLTR beta/TNFRSF3, rm4-1BB/TNFRSF9, rmCD27/TNFRSF7, rhCD30/TNFRSF8, rmCD40/TNFRSF5, rhDR3/TNFRSF12, rhDR6/TNFRSF21, rmFas/TNFRSF6, rmGITR/TNFRSF18, or rhHVEM/TNFRSF14 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CD30/TNFRSF8 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD30/TNFRSF8 Fc Chimera (Catalog # 852-CD)

Mouse CD30/TNFRSF8 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CD30/TNFRSF8 Biotinylated Antibody (Catalog # BAF852)
  • Standard: Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein, CF (Catalog # 852-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature mouse CD30 consists of a 264 amino acid (aa) extracellular domain (ECD) with three cysteine-rich repeats, a 27 aa transmembrane segment, and a 190 aa cytoplasmic domain (3). In contrast, human CD30 includes an additional 90 aa in the ECD and contains six cysteine-rich repeats. Within common regions of the ECD, mouse CD30 shares 53% and 80% aa sequence identity with human and rat CD30, respectively. CD30 is normally expressed on antigen‑stimulated Th cells and B cells (4‑6). However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling costimulates antigen‑induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL-13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85‑90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

References

  1. Kennedy, M.K. et al. (2006) Immunology 118:143.
  2. Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
  3. Bowen, M.A. et al. (1996) J. Immunol. 156:442.
  4. Hamann, D. et al. (1996) J. Immunol. 156:1387.
  5. Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
  6. Gruss, H.-J. et al. (1994) Blood 83:2045.
  7. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
  8. Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
  9. Harlin, H. et al. (2002) J. Immunol. 169:2451.
  10. Amakawa, R. et al. (1996) Cell 84:551.
  11. Chiarle, R. et al. (1999) J. Immunol. 163:194.
  12. Vinante, F. et al. (2002) Blood 99:52.
  13. Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
  14. Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
  15. Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.

Alternate Names

CD30, TNFRSF8

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Gene Symbol

TNFRSF8

UniProt

Q60846

Additional CD30/TNFRSF8 Products

Product Documents for Mouse CD30/TNFRSF8 Antibody

Product Datasheets

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Product Specific Notices for Mouse CD30/TNFRSF8 Antibody

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