Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB115F) or (B) Rat IgG_2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD5 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily (1). CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is a ligand of the B cell differentiation antigen CD72.

References

Ledbetter, J.A. and L.A. Herzenberg, (1979) Immunol. Rev. 47:63.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
Luo, W. et al. (1992) J. Immunol. 148:1630.
Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for Mouse CD5 Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD5 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD5 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB115F

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD5 Fluorescein-conjugated Antibody

Detection of CD5 in Mouse Splenocytes by Flow Cytometry.