Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 53-7.3

View all CD5 Antibodies »

Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Detects mouse CD5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CD5 PE-conjugated Antibody

Detection of CD5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD5 PE-conjugated Monoclonal Antibody (Catalog # FAB115P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD5 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a member of scavenger receptor cysteine-rich protein superfamily (1). CD5 is a membrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is a ligand of the B cell differentiation antigen CD72.

References

  1. Ledbetter, J.A. and L.A. Herzenberg, (1979) Immunol. Rev. 47:63.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for Mouse CD5 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD5 PE-conjugated Antibody

For research use only

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