D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

CD9 in Mouse Kidney.

CD9 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Mouse CD9 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse D3 cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a 24 kDa glycoprotein of the tetraspanin family that is expressed by a variety of hematopoietic and epithelial cells. It forms homotypic and heterotypic associations with other tetraspanin proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation. The extracellular portions of mouse CD9 share 81% and 88% amino acid identity with corresponding regions of human and rat CD9, respectively.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Entrez Gene IDs

928 (Human); 12527 (Mouse)

Gene Symbol

CD9

UniProt

P40240

Additional CD9 Products

Product Documents for Mouse CD9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD9 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5218

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD9 Antibody

Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry.