Mouse CD99 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3905
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6
Gly25-Leu138
Accession # Q8VCN6
Specificity
Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD99 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse CD99 Antibody
Detection of CD99 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (Catalog #NL002).Applications for Mouse CD99 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample:
Sample:
| Mouse splenocytes |
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD99
Sample: Recombinant Mouse CD99
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD99
CD99 (also known as PILR-L and MIC2) is a 125 amino acid, 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse CD99 protein used as immunogen is 45% identical to the human counterpart.
Alternate Names
CD99, MIC2, pilr-1, PILR-L
Gene Symbol
CD99
UniProt
Additional CD99 Products
Product Documents for Mouse CD99 Antibody
Product Specific Notices for Mouse CD99 Antibody
For research use only
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