Mouse Chk2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB22851
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 235303
Product Specifications
Immunogen
E. coli-derived recombinant mouse Chk2
Met1-Ala234
Accession # Q9Z265
Met1-Ala234
Accession # Q9Z265
Specificity
Detects endogenous mouse Chk2 in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Chk2 Antibody
Detection of Mouse Chk2 by Western Blot.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Chk2 Monoclonal Antibody (Catalog # MAB22851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chk2 at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Chk2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver and lung
Sample: Perfusion fixed frozen sections of mouse liver and lung
Western Blot
2 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line, under reducing conditions only
Sample: Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line, under reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk2
Checkpoint kinase 2 (Chk2) is a critical protein kinase involved in the cellular response to DNA damage. Chk2 is a target of the ATM protein kinase, and can phoshorylate multiple proteins, including Cdc25A and p53, to activate the G1/S cell cycle checkpoint. Patients with Li-Fraumeni syndrome, a cancer-prone familial disorder, may have a mutation in the chk2 or p53 genes, which suggests that Chk2 also functions as a tumor suppressor.
Long Name
Checkpoint Kinase 2
Alternate Names
CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53
Gene Symbol
CHEK2
UniProt
Additional Chk2 Products
Product Documents for Mouse Chk2 Antibody
Product Specific Notices for Mouse Chk2 Antibody
For research use only
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