Mouse Cyr61/CCN1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4864
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Functional Assay, Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 466305
Product Specifications
Immunogen
E. coli-derived recombinant mouse Cyr61/CCN1
Asp176-Gly281
Accession # P18406
Asp176-Gly281
Accession # P18406
Specificity
Detects mouse Cyr61/CCN1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cyr61 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Cyr61/CCN1 Antibody
Cyr61/CCN1 in Mouse Trigeminal Ganglia.
Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Cyr61/CCN1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse trigeminal ganglia
Sample: Perfusion fixed frozen sections of mouse trigeminal ganglia
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB4864 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyr61/CCN1
Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins alphaV beta3, alphaV beta5, alphaM beta2, and alpha6 beta1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Alternate Names
CCN1, GIG1, IGFBP-10
Gene Symbol
CCN1
UniProt
Additional Cyr61/CCN1 Products
Product Documents for Mouse Cyr61/CCN1 Antibody
Product Specific Notices for Mouse Cyr61/CCN1 Antibody
For research use only
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