Mouse Cystatin C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1238
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 207103
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C
aa 21-140
Accession # P21460
aa 21-140
Accession # P21460
Specificity
Detects recombinant mouse (rm) Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) Cystatin C is observed, but no cross-reactivity with rhCystatins A, D, S, SA or SN, rmCystatins B or EM, rhFetuin B, rmFetuin A, rhHPRG, or rhKininogen is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Cystatin C Antibody
Detection of Mouse Cystatin C by Western Blot.
Western blot shows lysates of mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Cystatin C Monoclonal Antibody (Catalog # MAB1238) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Cystatin C Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line
Sample: Mouse plasma and RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin C
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
UniProt
Additional Cystatin C Products
Product Documents for Mouse Cystatin C Antibody
Product Specific Notices for Mouse Cystatin C Antibody
For research use only
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