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Mouse
EpCAM/TROP-1 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8998R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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FAB8998R-100UG

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # G8.8R

Product Specifications

Immunogen

TE-71 Thymic epithelial cell line

Specificity

Detects mouse EpCAM/TROP-1 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EpCAM/TROP-1 Alexa Fluor® 647-conjugated Antibody

Detection of EpCAM/TROP-1 antibody in 4T1 Mouse Cell Line antibody by Flow Cytometry.

Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry.

4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8998R, filled histogram) or isotype control antibody (IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of EpCAM/TROP‑1 in mIMCD3 Mouse Cell Line by Flow Cytometry

mIMCD3 mouse kidney cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8998R, filled histogram) or isotype control antibody (IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse EpCAM/TROP-1 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: mIMCD3 mouse kidney cell line and 4T1 mouse breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as ECD326 and TROP-1 is a 36-41 kDa member of the TROP family of meolcules (1, 2). It is a type I transmembrane glycoprotein that is found on embryonic stem cells and adult non-squamous epithelium such as (respiratory) psuedostratified, (mammary) cuboid, and (colon) simple columnar epithelium. In mouse, the molecule has also been reported on T cells, Langerhans cells and thymic epithelium. Mature mouse EpCAM is 292 amino acids (aa) in length. It possesses a 243 aa extracellular region that contains one thyroglobulin type I domain. Such domains are associated with cathepsin binding and inhibition (2). On the cell surface, EpCAM is reported to participate in TEM (Tetraspanin-enriched microdomain) formation in concert with CD44v6, CD9, ADAM10, Claudin-7 and TS8. It is also noted to form homodimers in-cis and homotetramers in-trans. Although this would suggest the EpCAM acts as an adhesion molecule, in effect it does not, instead acting as a disruptor of the E-Cadherin:cytoskeleton interaction, and thus promoting cell migration (1, 2). The extracellular domain (ECD) of EpCAM undergoes multiple cleavages, principally between Ser200 and Ala210 (3). Cleavage at the Arg80-Arg81 site does not generate a soluble fragment, but rather a disulfide-linked "heterodimer" (3). Cleavage after Gly264 in the juxtamembrane region is followed by secretase cleavage of the transmembrane fragment, generating an ICD that translocates to the nucleus (4). The ECD of mouse EpCAM shares 81% and 90% aa sequence identity with human and rat EpCAM, respectively.

References

  1. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
  2. Martowicz, A. et al. (2015) Histol. Histopathol. Oct 23:11678 [ePub ahead of print].
  3. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
  4. Hachmeister, M. et al. (2013) PLoS ONE 8:e71836.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse EpCAM/TROP-1 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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