Mouse
EpCAM/TROP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8998

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB8998
MAB8998-SP
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-reported, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # G8.8R

View all EpCAM/TROP1 Antibodies »

Product Specifications

Immunogen

TE-71 Thymic epithelial cell line

Specificity

Detects mouse EpCAM/TROP-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EpCAM/TROP-1 Antibody

Detection of EpCAM/TROP-1 antibody in 4T1 Mouse Cell Line antibody by Flow Cytometry.

Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry.

4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB8998, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B).

Detection of EpCAM/TROP‑1 in mIMCD3 Mouse Cell Line by Flow Cytometry.

mIMCD3 mouse kidney cell line was stained with Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB8998, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B).

Applications for Mouse EpCAM/TROP-1 Antibody

Application
Recommended Usage

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352.

Flow Cytometry

0.25 µg/106 cells
Sample:  mIMCD3 mouse kidney cell line and 4T1 mouse breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as EGP314 (Epithelial glycoprotein 314), TACSTD1 (tumor-associated calcium signal transducer 1) and CD326 is a 292 amino acid (aa), 40 kDa transmembrane glycoprotein composed of a 243 aa extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (1). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (2). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha-actinin (3).

References

  1. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  2. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  3. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents for Mouse EpCAM/TROP-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EpCAM/TROP-1 Antibody

For research use only

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