Mouse
EpCAM/TROP-1 Antibody

Detection of EPCAM in Mouse Colon via seqIF™ staining on COMET™​

EPCAM was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse EPCAM, Monoclonal Antibody (Catalog # MAB11623) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37°Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EpCAM/TROP-1 in Mouse Colon.

EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB11623) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse EpCAM/TROP-1 by Western Blot.

Western Blot shows lysates of mouse colon tissue. PVDF membrane was probed with 4 µg/ml of Rat Anti-Mouse EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB11623) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.