Mouse F4/80 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5580
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 521204
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Gln28-Gly931
Accession # Q61549
Specificity
Detects mouse F4/80/EMR1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse F4/80 Antibody
Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).
F4/80 in RAW 264.7 mouse monocyte/macrophage cell line.
F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Applications for Mouse F4/80 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed and unfixed live RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed and unfixed live RAW 264.7 mouse monocyte/macrophage cell line
Reviewed Applications
Read 2 reviews rated 3 using MAB5580 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: F4/80
Alternate Names
DD7A5-7, Gpf480, Ly71
Gene Symbol
ADGRE1
UniProt
Additional F4/80 Products
Product Documents for Mouse F4/80 Antibody
Product Specific Notices for Mouse F4/80 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...