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Mouse Fc gamma RIII (CD16) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB19601

R&D Systems, part of Bio-Techne
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MAB19601
MAB19601-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

Bioassay, Flow Cytometry, Functional Assay, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 275003

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIII
Ala31-Thr215
Accession # Q5D5J5

Specificity

Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Fc gamma RIII (CD16) Antibody

Detection of Fc gamma RIII (CD16) antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB19601, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse Fc gamma RIII (CD16) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Gene Symbol

FCGR3

UniProt

Additional Fc gamma RIII (CD16) Products

Product Documents for Mouse Fc gamma RIII (CD16) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Fc gamma RIII (CD16) Antibody

For research use only

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