Mouse FGFR4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2265
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551
Leu19-Asp366
Accession # CAA42551
Specificity
Detects mouse FGF R4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) FGF R4 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) FGF R3 alpha (IIIb) is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse FGFR4 Antibody
Detection of Mouse FGF R4 by Western Blot.
Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF R4 at approximately 89 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FGF R4 in Mouse Embryo.
FGF R4 was detected in immersion fixed frozen sections of mouse embryo (11.5 d.p.c.) using Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse FGFR4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (11.5 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (11.5 d.p.c.)
Western Blot
0.25 µg/mL
Sample: Mouse heart tissue
Sample: Mouse heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using AF2265 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR4
References
- Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
- Cai, J. (2002) Dev. Biol. 251:221.
Long Name
Fibroblast Growth Factor Receptor 4
Alternate Names
CD334, FGF R4
Gene Symbol
FGFR4
UniProt
Additional FGFR4 Products
Product Documents for Mouse FGFR4 Antibody
Product Specific Notices for Mouse FGFR4 Antibody
For research use only
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