Mouse Frizzled-3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1001
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 169310
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086
Met1-Val157
Accession # Q61086
Specificity
Detects mouse Frizzled-3 in direct ELISAs and Western blots. In immunohistochemistry detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross‑reactivity with Frizzled-1, -4, -7 or -8 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Frizzled-3 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13-15)
Sample: Immersion fixed frozen sections of mouse embryo (E13-15)
Western Blot
1 µg/mL
Sample: Recombinant Mouse Frizzled-3
Sample: Recombinant Mouse Frizzled-3
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-3
Alternate Names
Frizzled3, FZD3
Gene Symbol
FZD3
UniProt
Additional Frizzled-3 Products
Product Documents for Mouse Frizzled-3 Biotinylated Antibody
Product Specific Notices for Mouse Frizzled-3 Biotinylated Antibody
For research use only
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