Detects mouse G-CSF R/CD114 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GM-CSF R alpha, rmM-CSF R, recombinant human (rh) G-CSF R alpha, or rhGM-CSF R beta is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse G-CSFR/CD114 Antibody

Detection of G-CSF R/CD114 in Mouse bone marrow by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (FAB1037P) and either (A) Rat Anti-Mouse G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB60391) or (B) Rat IgG2A Isotype Control (MAB006) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of G-CSF R/CD114 in Mouse splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat IgG2A Isotype Control (Catalog # MAB006) or (B) Rat Anti-Mouse G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB60391) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse G-CSFR/CD114 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse bone marrow cells and splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSFR/CD114

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

References

Ward, A.C. (2007) Front. Biosci. 12:608.
Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
Mitsui, T. et al. (2003) Blood 101:2990.
Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Entrez Gene IDs

1441 (Human); 12986 (Mouse)

Gene Symbol

CSF3R

UniProt

P40223

Additional G-CSFR/CD114 Products

Product Documents for Mouse G-CSFR/CD114 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse G-CSFR/CD114 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse G-CSFR/CD114 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB60391

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Mouse G-CSFR/CD114 Antibody

Immunogen

Specificity