Mouse GFR alpha-like Antibody

Detection of Mouse GFR alpha-like by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, mouse brain and mouse brain stem. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFRa-like at approximately 25 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GFR alpha-like in Mouse Spinal Cord.

GFR alpha-like was detected in immersion fixed paraffin-embedded sections of mouse spinal cord using Sheep Anti-Mouse GFR alpha-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with DAPI (blue). Specific staining was localized to dorsal and ventral horns. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

GFR alpha-like in Mouse Brain.

GFRa-like was detected in immersion fixed frozen sections of mouse brain using Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with Fluoro Nissl Green (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.