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Mouse IGFALS/ALS Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1436

R&D Systems, part of Bio-Techne
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MAB1436
MAB1436-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 199410

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389

Specificity

Detects mouse IGFALS/ALS in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IGFALS/ALS Antibody

Detection of Mouse IGFALS/ALS antibody by Western Blot.

Detection of Mouse IGFALS/ALS by Western Blot.

Western blot shows mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse IGFALS/ALS Monoclonal Antibody (Catalog # MAB1436) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for the ALS/IGFBP-3 Complex at approximately 140 kDa (as indicated) and ALS at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IGFALS/ALS Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFALS/ALS

Acid Labile Subunit (ALS) is a liver-derived serum protein that forms a ternary complex with IGFBP-3 and either IGF-I or IGF-II. The presence of ALS increases the half life of IGF/IGFBP-3 complexes in the circulation.

Long Name

IGF-binding Protein Acid Labile Subunit

Alternate Names

ACLSD, ALS

Entrez Gene IDs

3483 (Human); 16005 (Mouse)

Gene Symbol

IGFALS

UniProt

Additional IGFALS/ALS Products

Product Documents for Mouse IGFALS/ALS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IGFALS/ALS Antibody

For research use only

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